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HLAG 抗体 (APC)

HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC
产品编号 ABIN125708
发货至: 中国
  • 抗原 See all HLAG 抗体
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This HLAG antibody is conjugated to APC
    应用范围
    • 85
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    Flow Cytometry (FACS)
    特异性
    The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
    无交叉反应
    小鼠
    交叉反应 (详细)
    Human
    纯化方法
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    免疫原
    Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
    克隆位点
    MEM-G-9
    亚型
    IgG1
    Top Product
    Discover our top product HLAG Primary Antibody
  • 应用备注
    Flow cytometry: Recommended dilution: 1-5 μg/mL, positive control: JEG-3 human choriocarcinoma cell line.
    说明

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    Avoid prolonged exposure to light.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Zhao, Teklemariam, Hantash: "Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies." in: Tissue antigens, Vol. 80, Issue 3, pp. 231-8, (2012) (PubMed).

    López, Alegre, LeMaoult, Carosella, González: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." in: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, (2006) (PubMed).

    Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).

    Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, (2003) (PubMed).

    Lozano, González, Kindelán, Rouas-Freiss, Caballos, Dausset, Carosella, Peña: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." in: AIDS, Vol. 16, Issue 3, pp. 347-51, (2002) (PubMed).

    Pangault, Le Friec, Caulet-Maugendre, Léna, Amiot, Guilloux, Onno, Fauchet: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." in: Human immunology, Vol. 63, Issue 2, pp. 83-90, (2002) (PubMed).

    Fuzzi, Rizzo, Criscuoli, Noci, Melchiorri, Scarselli, Bencini, Menicucci, Baricordi: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." in: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, (2002) (PubMed).

    Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, (2000) (PubMed).

  • 抗原
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    别名
    HLA-G (HLAG 产品)
    别名
    MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
    背景
    Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
    基因ID
    3135
    UniProt
    P17693
    途径
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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