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CD137 抗体 (AA 101-200)

Name: CD137 抗体 (AA 101-200)
SKU: ABIN740445
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-CD137 antibody is a 兔多克隆 antibody detecting CD137 in ELISA, FACS, WB 和 IHC (p). Suitable for 人, 小鼠和大鼠. Independently validated for use in Flow Cytometry. This Primary Antibody has been cited in 2+ publications.

Flow Cytometry validation image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 101-200) antibody (ABIN740445) (CD137 抗体 (AA 101-200))

FACS

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD137/TNFRSF9 Polyclonal Antibody, Unconjugated (ABIN740445) at 1:600 followed by conjugation to the secondary antibody (CD137 抗体 (AA 101-200))

IHC

2 images

(2 references)

(1 validation)

产品编号 ABIN740445

发货至: 中国

Quick Overview for CD137 抗体 (AA 101-200) (ABIN740445)

抗原

See all CD137 (TNFRSF9) 抗体

CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CD137 antibody is un-conjugated

应用范围

ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

抗原表位
15

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6

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3

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1

1

1

1

1

1

1

1

1
AA 101-200

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD137

亚型

IgG
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 19-188) antibody
TNFRSF9 适用: 人 ELISA, FACS, WB, IHC, IF, IHC (p), Coat, StM 宿主: 小鼠 Monoclonal 4-1BB-3201 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 214-255) antibody
TNFRSF9 适用: 人 ELISA, FACS, WB 宿主: 小鼠 Monoclonal 2A12C2 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 40-153) antibody
TNFRSF9 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) antibody
TNFRSF9 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM66 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 150-250) antibody
TNFRSF9 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) antibody
TNFRSF9 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4G10B9 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibody
TNFRSF9 适用: 人, Non-Human Primate ELISA, FACS, IP 宿主: 小鼠 Monoclonal 4B4-1 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibody
TNFRSF9 适用: 人, Non-Human Primate ELISA, FACS, Func, IP 宿主: 小鼠 Monoclonal 4B4-1 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (Internal Region) antibody
TNFRSF9 适用: 人, 大鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibody
TNFRSF9 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

限制

仅限研究用
生效 #101793 (Flow Cytometry)

by

Pharmacology Department, Faculty of Medicine, Granada University

No.

#101793

日期

2017.09.13

抗原

CD137

Lot Number

AG05152084

Method validated

Flow Cytometry

Positive Control

rat adipose-derived mesenchymal stem cells

Negative Control

cells stained with secondary antibody only

Notes

Passed. ABIN740445 specifically recognizes Cd137 in rat adipose-derived mesenchymal stem cells.

Validation Images

Flow cytometric analysis of cells stained with ABIN740445 and an AF680-conjugated secondary antibody (A) or the secondary antibody only (B). The top row illustrates the gating for life cells. The second and third row show dots blots and histograms respectively of the positively stained cell populations.
Full Methods
Primary Antibody

ABIN740445

Secondary Antibody

donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322)

Full Protocol

Grow rat adipose-derived mesenchymal stem cells in DMEM high glucose, GLUTAMAX, pyruvate supplemented (Gibco, 31966021, lot 1774921) supplemented with 10% fetal bovine serum (Gibco, 16000044, lot 1774921) 1% penicillin/streptomycin (Gibco, 15140122, lot 1759446), at 37°C and 5% CO₂ in ml on a flask to 95% confluency.
Wash cells with PBS buffer.
Detach cells from the flask with trypsin-versene solution (Lonza, H3BE17-161E, lot RB35937) for 3min at 37°C and 5% CO₂.
Centrifuge cells for at 400xg for 5min at RT, aspirate the supernatant.
Resuspend cells in 1mL of 1% BSA in PBS per 2x10⁶ cells and incubate 20min at RT.
Incubate cells with primary rabbit anti-CD137 antibody (antibodies-online, ABIN740445, lot AG05152084) diluted 1:35 in 1% BSA in PBS buffer for 20min on ice. Include a negative control without primary antibody.
Wash cells 1x with PBS buffer and centrifuge them for 400xg for 5min at RT.
Resuspend cells in 175μl 1% BSA in PBS buffer.
Incubate cells with secondary donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322) diluted 1:200 in buffer for 15min in ice.
Wash cells 2xwith PBS buffer and centrifuge them for 400xg for 5min at RT.
Resuspend the pellet in 500μl PBS buffer.
Data acquisition in a BD FACSCanto II flow cytometer, equipped with two laser lines of 488nm and 633nm and two scattered light detectors (FSC and SSC). Detect fluorescence in PMTs with filters suitable for the fluorescence of the fluorophore AF680 (735LP and 780/60BP). Threshold is established in FSC. The data is saved in files in FCS format and analyzed offline with the FACSDiva software (BD).

Experimental Notes

ABIN740445 reveals a specific fluorescence signal in rat adipose-derived mesenchymal stem cells. This is the first time that flow cytometry is performed in this type of cells using this antibody. No signal was registered in the negative control cells.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Claycombe, Vomhof-DeKrey, Garcia, Johnson, Uthus, Roemmich et al.: "Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, (2016) (PubMed).

Daquinag, Tseng, Salameh, Zhang, Amaya-Manzanares, Dadbin, Florez, Xu, Tong, Kolonin: "Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development." in: Cell death and differentiation, (2014) (PubMed).
抗原

CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

别名

CD137

背景

Synonyms: ILA, 4-1BB, CD137, CDw137, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9
Background: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

基因ID

3604

UniProt

Q07011

途径

Cancer Immune Checkpoints