Nestin 抗体
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北京 101111
Quick Overview for Nestin 抗体 (ABIN7467398)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 人, 兔
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Nestin. The exact sequence is proprietary.
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亚型
- IgG
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 IHC, WB, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 IHC, WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1392-1621) antibodyNES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: SH-SY-5Y , SK-N-SH
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.38 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Nestin (NES)
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别名
- nestin
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背景
- Nestin , Nbla00170,Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
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分子量
- 177 kDa
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基因ID
- 10763
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UniProt
- P48681
抗原
-
