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Nestin 抗体 (C-Term)

This 小鼠 单克隆 anti-Nestin antibody (Clone GT623) specifically detects Nestin in WB, ICC, IF, IHC (p) 和 IHC (fro). The antibody is reactive with 人 samples.
产品编号 ABIN7464939
发货至: 中国
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Quick Overview for Nestin 抗体 (C-Term) (ABIN7464939)

抗原

See all Nestin (NES) 抗体
Nestin (NES)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Nestin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

GT623
  • 抗原表位

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    C-Term

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.

    亚型

    IgG1
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: SK-N-SH , IMR32

    Validation: Comparison, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1XPBS pH 7, 20 % Glycerol, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    Nestin (NES)

    别名

    nestin

    背景

    Nestin , Nbla00170,Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

    分子量

    177 kDa

    基因ID

    10763

    UniProt

    P48681
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