MRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange
纯化方法
Produced in: E.coli
免疫原
Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.
RFP
适用: Discosoma
WB, ELISA, IF
宿主: 兔
Polyclonal
HRP
应用备注
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconsitute with 250 µL pure water for immidate use
缓冲液
sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.
储存条件
4 °C/-20 °C
储存方法
Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.