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DYKDDDDK Tag 抗体

WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4G5C8 unconjugated

Immunoprecipitating Flag Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG instead of in Flag Tag in 293F transfected whole cell lysate. (DYKDDDDK Tag 抗体)

Western Blot Positive WB detected in: 10 ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa (DYKDDDDK Tag 抗体)

Western Blot Positive WB detected in: Flag Tag fusion protein at 50 ng, 25 ng, 12. (DYKDDDDK Tag 抗体)

Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate Lane 1: Mouse control IgG instead of in Transfected HEK-293 cell whole cell lysate Lane 2: (1 μL) + Transfected HEK-293 cell whole cell lysate (500 μg) Lane 3: Transfected HEK-293 cell whole cell lysate (20 μg) For western blotting, the blot was detected with at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 (DYKDDDDK Tag 抗体)

Western Blot Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50 ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 59. (DYKDDDDK Tag 抗体)

Immunofluorescence staining of 293T cells transfected with Flag tag by at 1:300, counter-stained with DAPI. (DYKDDDDK Tag 抗体)

6 images

产品编号 ABIN7322021

发货至: 中国

Datasheet as PDF

抗原 See all DYKDDDDK Tag products

DYKDDDDK Tag

适用

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宿主
57

51

8

6

6
小鼠
Compatible Secondaries
克隆类型
64

63
单克隆
标记
45

15

10

7

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DYKDDDDK Tag antibody is un-conjugated
应用范围
78

61

28

23

19

17

12

12

10

6

5

4

3

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

纯化方法

Protein G purified

免疫原

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

克隆位点

4G5C8

亚型

IgG2a
anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
D 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP 宿主: 兔 Polyclonal HRP

anti-DYKDDDDK Tag antibody
IP 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF 宿主: 小鼠 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA 宿主: 小鼠 Monoclonal 2F11G1 unconjugated

应用备注

Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5 μL-2 μL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

DYKDDDDK Tag

别名

Flag Tag (DYKDDDDK Tag 产品)

物质类

Tag

背景

Background: Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Aliases: DDK tag, DYKDDDDK, Flag, Flag Tag

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