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DYKDDDDK Tag 抗体

WB, IP, IF 宿主: 小鼠 unconjugated
产品编号 ABIN1112984
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    适用
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    宿主
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    小鼠
    标记
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    This DYKDDDDK Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    序列
    DYKDDDDK
    免疫原
    A synthetic peptide DYKDDDDK conjugated to KLH.
    亚型
    IgG1
  • 应用备注
    WB (1:1000-10000), IP (1:100-500), IF (1:200-1000). Other applications have not been tested. The optimal dilutions should be determined by end user.
    限制
    仅限研究用
  • 生效 #028889 (Immunofluorescence)
    '独立验证'标志
    by
    Reveal Biosciences
    No.
    #028889
    日期
    2013.11.07
    抗原
    Lot Number
    L21/2013G
    Method validated
    Immunofluorescence
    Positive Control
    HEK293 cells transfected with DYKDDDDK Tag
    Negative Control
    Untransfected HEK293 cells
    Notes
    Signal was detected in positive control samples but not in negative control samples.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: DYKDDDDK Tag antibody
    • Catalog number: ABIN1112984
    • Lot number: L21/2013G
    Secondary Antibody
    • Antibody: Goat anti-mouse IgG (H+L) IF488 conjugated antibody
    • Lot number: S6/2013S
    Full Protocol
    • HEK293 cells were grown directly on coverslips and transfected with a DYKDDDDK tagged protein.
    • Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1 X PBS / 5% normal goat serum / 0.3% Triton X-100 for 20 min at RT.
    • Cells were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron
    • Microscopy Sciences, 25885-05) overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:150 in Universal Antibody Dilution Buffer
    • (Electron Microscopy Sciences, 25885-05) for 60 min at RT in dark.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with Fluoro-Gel II with DAPI (Electron Microscopy Sciences Cat. No: 17985-50).
    • Stained cells were imaged with an EIDAQ 100 fluorescent microscope.
    Experimental Notes
    None
  • 状态
    Liquid
    缓冲液
    PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
    储存液
    Sodium azide, Thimerosal (Merthiolate)
    储存条件
    4 °C
    储存方法
    Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
  • 抗原
    DYKDDDDK Tag
    别名
    Flag Tag (DYKDDDDK Tag 产品)
    物质类
    Tag
    背景
    DYKDDDDK-Tag, or DYKDDDDK octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the DYKDDDDK-Tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.
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