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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Immunoprecipitation (IP)
- 序列
- DYKDDDDK
- 特异性
- This product is a monoclonal antibody coupled to activated agarose. This antibody is directed against the DYKDDDDK epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the DYKDDDDK epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- 产品特性
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- 纯化方法
- purified
- 过滤
- Sterile filtered
- 免疫原
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This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type: Peptide - 克隆位点
- 29E4-G7
- 亚型
- IgG2a kappa
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4G5C8 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyD 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
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- 应用备注
- Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of DYKDDDDK tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the DYKDDDDK epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.
- 说明
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1 mL agarose-conjugated antibody in 1.5 mL buffer
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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- 抗原
- DYKDDDDK Tag
- Abstract
- DYKDDDDK Tag 产品
- 物质类
- Tag
- 背景
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Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, DYKDDDDK and HA.
Synonyms: DYKDDDDK tag, DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K
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