MCM2 抗体 (AA 650-750)
Quick Overview for MCM2 抗体 (AA 650-750) (ABIN7126677)
抗原
See all MCM2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 650-750
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特异性
- The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (aa 650-750) of human MCM2 protein (exact sequence is proprietary)
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亚型
- IgG1
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anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibody
MCM2 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibodyMCM2 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E7 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 805-904) antibodyMCM2 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6A8 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibodyMCM2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 884-904) antibodyMCM2 适用: 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (N-Term) antibodyMCM2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 360-700) antibodyMCM2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 889-904), (C-Term) antibodyMCM2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 884-904), (C-Term) antibodyMCM2 适用: 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 649-677) antibodyMCM2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, Jurkat or Ramos cells. Human tonsil.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
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别名
- MCM2
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背景
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BM28, CCNL1, CDC like 1, cdc19, CDCL1, Cell devision cycle like 1, Cyclin like 1, DNA replication licensing factor MCM2, KIAA0030, Minichromosome maintenance complex component 2, Minichromosome maintenance deficient 2 (mitotin), Minichromosome maintenance protein 2 homolog, Mitotin, Nuclear protein BM28,MCM2 (ProliferationMarker)
Cellular localisation: Nuclear -
分子量
- 102kDa
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基因ID
- 4171, 477481
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UniProt
- P49736
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
-
