SLC12A1 抗体 (Intracellular)
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Quick Overview for SLC12A1 抗体 (Intracellular) (ABIN7043674)
抗原
See all SLC12A1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 776-790, Intracellular
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原理
- A Rabbit Polyclonal Antibody to NKCC2 Transporter
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特异性
- Intracellular, C-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 13,15 amino acid residues identical
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产品特性
- Anti-NKCC2 (SLC12A1) (extracellular) Antibody is directed against an epitope of rat NKCC2 transporter. Anti-NKCC2 (SLC12A1) (extracellular) Antibody (ABIN7043674, ABIN7044815 and ABIN7044816). The antibody recognizes an extracellular epitope and is thus ideal for detecting NKCC2 in living cells. It has been designed to recognize NKCC2 from rat, human and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)GYKKNWRKAPLSELE, corresponding to amino acid residues 776-790 of rat NKCC2
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亚型
- IgG
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anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (N-Term) antibody
SLC12A1 适用: 人, 大鼠, 小鼠, 犬, Pig, 兔, 豚鼠, Cow, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) antibodySLC12A1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibodySLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 52-83), (N-Term) antibodySLC12A1 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) antibodySLC12A1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB945 unconjugated Recombinant Antibody
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) antibodySLC12A1 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 80-172) antibodySLC12A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 351-400) antibodySLC12A1 适用: 人, 大鼠, 小鼠, 猴, 犬, Pig, 兔, Cow, 马, Bat WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (HRP)SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal HRP
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (FITC)SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7236937)
Blocking Peptide: (ABIN7236937)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))
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别名
- SLC12A1
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背景
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Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1,Na+-K+-2Cl- cotransporter (NKCC2) belongs to the family of integral plasma membrane proteins, solute carrier family 12 ,the electrically silent, cation-coupled chloride co-transporter family that mediates electroneutral symport of Na+, K+, and Cl- with a stochiometry of 1:1:2, respectively. NKCC2 structure contains a central hydrophobic domain made up of 12 putative transmembrane spanning regions and a long hydrophilic loop connects transmembrane segments 7 and 8 and contains two glycosylation sites. The hydrophobic domain is flanked by a short amino- and a long carboxyl-terminal domain that are located within the cell.NKCC2 encoded by the Slc12a1 gene shows at least six alternatively spliced variants and has the ability to form dimmers. NKCC2 activity and expression can be found in the medullary and cortical thick ascending limb of Henle's loop is the nephron and in macula densa cells.NKCC2 allows Na+ and Cl- entry from the tubule lumen into the thick ascending limb of Henle's loop, a process that allows reabsorbing 25-30 % of the NaCl filtered by the glomeruli. In addition, NKCC2 can sense luminal Cl- concentration and initiates the signal for tubuloglomerular feedback in the specialized cells of the macula densa. Thus, NKCC2 activity is essential for salt conservation and for acute and chronic regulation of water balance.Mutations in the human NKCC2 gene causes Bartter syndrome type 1 characterized by severe natriuresis hypercalciuria and magnesuria, the inability to concentrate urine, leading to hyponatremia, hypochloremia, metabolic alkalosis, and low blood pressure. Opposite to Bartter's syndrome, enhanced activity of NKCC2 NaCl is connected with human and animal hypertension.
Alternative names: NKCC2 (SLC12A1), Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1 -
基因ID
- 25065
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NCBI登录号
- NM_000338
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UniProt
- P55016
抗原
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