SLC12A1 抗体 (AA 33-55)
(4 references)-
- 抗原 See all SLC12A1 抗体
- SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))
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抗原表位
- AA 33-55
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC12A1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Detects ~160 kDa.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein A Purified
- 免疫原
- Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
- Top Product
- Discover our top product SLC12A1 Primary Antibody
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anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (N-Term) antibody
SLC12A1 适用: 大鼠, 人, 小鼠, 犬, Pig, 兔, 豚鼠, Cow, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) antibodySLC12A1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 52-83), (N-Term) antibodySLC12A1 适用: 大鼠, 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) antibodySLC12A1 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 80-172) antibodySLC12A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 351-400) antibodySLC12A1 适用: 大鼠, 人, 小鼠, 猴, 犬, Pig, 兔, Cow, 马, Bat WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (HRP)SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal HRP
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (FITC)SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal FITC
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (Alkaline Phosphatase (AP))SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1) (AA 33-55) antibody (Biotin)SLC12A1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal Biotin
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- 应用备注
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- WB (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN863200 was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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: "Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways." in: Journal of the American Heart Association, Vol. 6, Issue 10, (2018) (PubMed).
: "Regulation of the Na(+) -K(+) -2Cl(-) cotransporter by cGMP/cGMP-dependent protein kinase I after furosemide administration." in: The FEBS journal, (2015) (PubMed).
: "Inhibition of Mitochondrial Complex-1 Prevents the Downregulation of NKCC2 and ENaCα in Obstructive Kidney Disease." in: Scientific reports, Vol. 5, pp. 12480, (2015) (PubMed).
: "Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK." in: American journal of physiology. Renal physiology, Vol. 307, Issue 1, pp. F96-F106, (2014) (PubMed).
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: "Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways." in: Journal of the American Heart Association, Vol. 6, Issue 10, (2018) (PubMed).
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- 抗原
- SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))
- 别名
- NKCC2 (SLC12A1 产品)
- 别名
- slc12a1 antibody, SLC12A1 antibody, DKFZp469A2020 antibody, BSC1 antibody, NKCC2 antibody, Nkcc2 antibody, AI788571 antibody, D630042G03Rik antibody, mBSC1 antibody, urehr3 antibody, si:ch211-220f12.1 antibody, solute carrier family 12 member 1 antibody, solute carrier family 12, member 1 antibody, si:ch211-220f12.1 antibody, SLC12A1 antibody, Slc12a1 antibody
- 背景
- NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
- 基因ID
- 25065
- NCBI登录号
- NP_001257546
- UniProt
- Q5PQV1
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