ERK2 抗体 (AA 25-360)
Quick Overview for ERK2 抗体 (AA 25-360) (ABIN7445753)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-360
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原理
- Polyclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)
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特异性
- The antibody is a rabbit polyclonal antibody raised against ERK2. It has been selected for its ability to recognize ERK2 in immunohistochemical staining and western blotting.
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交叉反应
- 小鸡, Cow, 犬, 山羊, 豚鼠, 小鼠, Pig, 大鼠, 绵羊
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Extracellular Signal Regulated Kinase 2 (ERK2) corresdonding to Tyr25~Ser360 with N-terminal His Tag
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亚型
- IgG
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应用备注
- Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin, Sodium azide
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注意事项
- This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- ERK2
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背景
- MAPK1, P38, P40, ERK2, ERT1, MAPK2, P42MAPK, P42-MAPK, PRKM1, PRKM2, P41, P41mapk, P41-mapk, Mitogen-Activated Protein Kinase 1, Mitogen-activated protein kinase 2
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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