TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
纯化方法
TRPV4 antibody is affinity chromatography purified via peptide column.
免疫原
TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4. The immunogen is located within amino acids 380 - 430 of TRPV4.
TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
TRPV4 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
TRPV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).