This Wnt10a antibody is predicted to not cross-react with Wnt10b.
纯化方法
Wnt10a Antibody is affinity chromatography purified via peptide column.
免疫原
Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300 - 350 of Wnt10a.
Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Wnt10a Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
Wnt10a antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
WNT10A
(Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))
WNT10A antibody, wnt10a/12 antibody, wnt10a antibody, OODD antibody, SSPS antibody, STHAG4 antibody, WNT-10A antibody, Wnt family member 10A antibody, protein Wnt-10a antibody, Wnt10a protein antibody, hypothetical protein antibody, WNT10A protein antibody, Wnt family member 10A L homeolog antibody, wingless-type MMTV integration site family, member 10A antibody, wingless-type MMTV integration site family, member 10a antibody, WNT10A antibody, LOC778792 antibody, CpipJ_CPIJ005839 antibody, Wnt10a antibody, wnt10a antibody, wnt10a.L antibody
背景
Wnt10a Antibody: Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.