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CD45 抗体 (redFluor™ 710)

PTPRC 适用: 人 FACS 宿主: 小鼠 Monoclonal HI30 redFluor™ 710
产品编号 ABIN7278599
发货至: 中国
  • 抗原 See all CD45 (PTPRC) 抗体
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    适用
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    小鼠
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    单克隆
    标记
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    This CD45 antibody is conjugated to redFluor™ 710
    应用范围
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    Flow Cytometry (FACS)
    交叉反应
    Chimpanzee
    纯化方法
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    克隆位点
    HI30
    亚型
    IgG1 kappa
    Top Product
    Discover our top product PTPRC Primary Antibody
  • 应用备注
    This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. redFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. redFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.
    说明

    5 uL (0.25 ug)/test

    限制
    仅限研究用
  • 缓冲液
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    2-8°C protected from light
    有效期
    12 months
  • 抗原
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    别名
    CD45 (PTPRC 产品)
    别名
    PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
    背景
    The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.
    基因ID
    5788
    UniProt
    P08575
    途径
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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