CD73 抗体
-
- 抗原 See all CD73 (NT5E) 抗体
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD73 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant full-length human NT5E protein
- 克隆位点
- NT5E-2646
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NT5E Primary Antibody
-
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Positive Control: A431, U87MG, PC-3 cells. Tonsil, Prostate or Lung Carcinoma.
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells in 0.1ml),Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- 别名
- NT5E (NT5E 产品)
- 别名
- CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody
- 背景
- CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.
- 分子量
- 71kDa
- 基因ID
- 4907
- UniProt
- P21589
- 途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
-
