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CD73 抗体 (C-Term)

Name: CD73 抗体 (C-Term)
SKU: ABIN388750
Availability: OutOfStock

NT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated

Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (ABIN388750 and ABIN2839011).

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term) (ABIN388750 and ABIN2839011) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

IHC (p)

Western blot analysis of CD73 Pab (ABIN388750 and ABIN2839011) in mouse brain tissue lysate.

Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E- Antibody (ABIN388750 and ABIN2839011).

All lanes : Anti-NT5E Antibody at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 μg per lane.

5 images

(10 references)

产品编号 ABIN388750

发货至: 中国

Datasheet as PDF

抗原 See all CD73 (NT5E) 抗体

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
抗原表位
16

9

8

6

5

5

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 520-550, C-Term
适用
101

50

26

18

18

16

4

2

1

1

1

1

1
人, 小鼠
宿主
81

31

9

2
兔
Compatible Secondaries
克隆类型
72

51
多克隆
标记
58

14

11

7

7

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD73 antibody is un-conjugated
应用范围
83

52

48

40

17

14

14

14

12

7

5

3

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

克隆位点

RB02846

亚型

Ig Fraction

Top Product

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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人 WB, FACS, IHC, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibody
NT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibody
NT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Soleimanifar, Mortazavi, Nadri, Soleimani: "Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 10, pp. 2703-2711, (2018) (PubMed).

Yu, Wang, Lu, Wang, Li, Liao, Zhu, Lv, Zhi, Yu, Jin, Zou, Ou, Liu, Zhou: "Extracellular 5'-nucleotidase (CD73) promotes human breast cancer cells growth through AKT/GSK-3β/β-catenin/cyclinD1 signaling pathway." in: International journal of cancer, Vol. 142, Issue 5, pp. 959-967, (2018) (PubMed).

Tak, Jung, Kim, Park, Jun, Lee, Jung, Kirchner, Hwang, Song, Lee: "Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure." in: Toxicology and applied pharmacology, Vol. 314, pp. 72-81, (2017) (PubMed).

Cartón-García, Overeem, Nieto, Bazzocco, Dopeso, Macaya, Bilic, Landolfi, Hernandez-Losa, Schwartz, Ramon Y Cajal, van Ijzendoorn, Arango: "Myo5b knockout mice as a model of microvillus inclusion disease." in: Scientific reports, Vol. 5, pp. 12312, (2015) (PubMed).

Zhi, Wang, Yu, Yu, Zhang, Yin, Zhou: "Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer." in: IUBMB life, Vol. 64, Issue 11, pp. 911-20, (2012) (PubMed).

Hart, Grenz, Gorzolla, Schittenhelm, Dalton, Eltzschig: "Hypoxia-inducible factor-1?-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4367-74, (2011) (PubMed).

Zhi, Wang, Zhou, Yu, Jian, Tang, Yin, Zhou: "RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells." in: Cancer science, Vol. 101, Issue 12, pp. 2561-9, (2010) (PubMed).

Leth-Larsen, Lund, Hansen, Laenkholm, Tarin, Jensen, Ditzel: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, (2009) (PubMed).

Hart, Henn, Köhler, Kloor, Mittelbronn, Gorzolla, Stahl, Eltzschig: "Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2784-97, (2008) (PubMed).

Macdonald, Pike: "A simplified method for the preparation of detergent-free lipid rafts." in: Journal of lipid research, Vol. 46, Issue 5, pp. 1061-7, (2005) (PubMed).
抗原

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

别名

CD73 (NT5E) (NT5E 产品)

别名

CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

背景

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

分子量

63368

基因ID

4907

NCBI登录号

NP_001191742, NP_002517

UniProt

P21589

途径

Synaptic Membrane, Ribonucleoside Biosynthetic Process