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HSP27 抗体

HSPB1 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal CPTC-HSPB1-2 unconjugated
产品编号 ABIN6939664
发货至: 中国
  • 抗原 See all HSP27 (HSPB1) 抗体
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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    小鼠
    克隆类型
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    单克隆
    标记
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    This HSP27 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant human full-length protein
    克隆位点
    CPTC-HSPB1-2
    亚型
    IgG1 kappa
    Top Product
    Discover our top product HSPB1 Primary Antibody
  • 应用备注

    Positive Control: MCF-7, HeLa cells, ~50 % of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    别名
    HSPB1 (HSPB1 产品)
    别名
    CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
    背景
    HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
    分子量
    27kDa
    基因ID
    3315
    UniProt
    P04792
    途径
    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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