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HSP27 抗体 (AA 101-205)

Name: HSP27 抗体 (AA 101-205)
SKU: ABIN672441
Availability: OutOfStock

HSPB1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (ABIN672441) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin embedded rat brain labeled with (ABIN672441) Rabbit Anti-HSP27 HspB1 HSP25 Polyclonal Antibody, unconjugated 1: 200 followed by incubation with conjugated secondary antibody and DAB staining.

IHC

A431 cells probed with HSP27 Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (ABIN672441) at 1:200 overnight at 4 °C.

SDS

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (ABIN672441) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC (p)

6 images

(3 references)

产品编号 ABIN672441

发货至: 中国

Datasheet as PDF

抗原 See all HSP27 (HSPB1) 抗体

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
抗原表位
41

36

27

16

15

10

8

8

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-205
适用
295

103

89

24

14

14

13

12

11

6

6

4

3

2

2

2

1
人, 大鼠, 小鼠
宿主
233

66
兔
Compatible Secondaries
克隆类型
205

93
多克隆
标记
134

23

17

13

12

8

7

7

7

7

7

7

4

4

4

4

4

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
应用范围
252

103

102

94

68

49

49

45

40

39

15

12

10

7

7

2

2

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human HSP27

亚型

IgG
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人, 大鼠, 小鼠, 绵羊, 猴, 小鸡, Chimpanzee WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal G3-1 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibody
HSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
HSPB1 适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 小鼠 Monoclonal 5D12-A12 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (FITC)
HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 FITC

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Feng, Wang, Dong, Gong, Pu, Chen: "Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 5246-5253, (2018) (PubMed).

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, (2014) (PubMed).

Wang, Yang, Liu, Zhou, Wu, Qiao, Li, Wang: "Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets." in: The Journal of nutrition, Vol. 142, Issue 1, pp. 7-13, (2011) (PubMed).
抗原

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

别名

HSP27 (HSPB1 产品)

别名

CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

背景

Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.7667, HEL-S-12, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27
Background: Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

基因ID

3315

UniProt

P04792

途径

MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling