XRCC6/XRCC5 抗体
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- 抗原 See all XRCC6/XRCC5 products
- XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This XRCC6/XRCC5 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 特异性
- Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
- 无交叉反应
- 小鸡, 小鼠, 大鼠
- 交叉反应 (详细)
- Human. Primates. Does not react with mouse, rat and chicken.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Nuclear extract of human HL-60 cells
- 克隆位点
- KU729
- 亚型
- IgG1 kappa
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunocytochemistry (Acetone-fixed cells) (0.5-1.0 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: K562, HL-60, HEp-2 or HeLa cells.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))
- 别名
- XRCC6 (p70) & XRCC5 (p80) (XRCC6/XRCC5 产品)
- 背景
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70 kDa / 80 kDa subunit of Ku antigen, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6), ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, KARP1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), X-ray repair cross-complementing protein 5 (XRCC5),Ku (p70/p80) (Nuclear Marker)
Cellular localisation: Nuclear - 分子量
- 70kDa & 80kDa
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