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CD45 抗体

PTPRC 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal UCHL-1-T200-797 unconjugated
产品编号 ABIN6938269
发货至: 中国
  • 抗原 See all CD45 (PTPRC) 抗体
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD45 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1), Human T-lymphocytes (T200/797)
    克隆位点
    UCHL-1-T200-797
    亚型
    IgG2a kappa
    Top Product
    Discover our top product PTPRC Primary Antibody
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: CCRF-CEM, Jurkat or MOLT-4 cells. Human Spleen, Paracortex in a tonsil or a reactive lymph node.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    别名
    PTPRC (PTPRC 产品)
    别名
    PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
    背景
    T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein,CD45RO (T-Cell Marker)
    Cellular localisation: Cell surface
    分子量
    180-185kDa
    基因ID
    5788, 654514
    UniProt
    P08575
    途径
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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