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BAK1 抗体 (AA 21-120)

Name: BAK1 抗体 (AA 21-120)
SKU: ABIN674409
Availability: OutOfStock

BAK1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Lane 1: Mouse lung lysate probed with Rabbit Anti-BAK Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (BAK1 抗体 (AA 21-120))

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Bak Polyclonal Antibody, Unconjugated (ABIN674409) followed by conjugation to the secondary antibody and DAB staining (BAK1 抗体 (AA 21-120))

IHC

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-BAK Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (BAK1 抗体 (AA 21-120))

IHC (p)

3 images

(3 references)

产品编号 ABIN674409

发货至: 中国

Datasheet as PDF

抗原 See all BAK1 抗体

BAK1 (BCL2-Antagonist/killer 1 (BAK1))
抗原表位
19

15

8

7

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 21-120
适用
77

31

22

15

3

2

1

1

1

1
人, 小鼠, 大鼠, 小鸡
宿主
71

4

2

1
兔
Compatible Secondaries
克隆类型
63

15
多克隆
标记
44

5

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1
This BAK1 antibody is un-conjugated
应用范围
67

27

26

18

13

13

13

12

8

7

4

3

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鸡, 人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse BAK

亚型

IgG

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anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody
BAK1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Antagonist/killer 1 (BAK1) antibody
BAK1 适用: 人 WB, ELISA, IHC, FACS, IP 宿主: 兔 Monoclonal 8D1 unconjugated Recombinant Antibody

anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody
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anti-BCL2-Antagonist/killer 1 (BAK1) antibody
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anti-BCL2-Antagonist/killer 1 (BAK1) (AA 1-14) antibody
BAK1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Antagonist/killer 1 (BAK1) antibody
BAK1 适用: 人, Hamster, 猴, Pig WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Antagonist/killer 1 (BAK1) (N-Term) antibody
BAK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Antagonist/killer 1 (BAK1) (N-Term) antibody
BAK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Yang, Pei, Zhang, Liao, Yu, Qiao, Han, Li, Hu, Guo, Pan, Tang: "Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 54, pp. 310-316, (2019) (PubMed).

Huang, Chao, Lee, Lu, Cheng, Yang, Wang, Chang, Huang: "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." in: Molecular neurobiology, (2015) (PubMed).

Zhu, Wang, Liang, Wang, Zhang, Li, Ying: "Regulation of cell transformation by Rb-controlled redox homeostasis." in: PLoS ONE, Vol. 9, Issue 7, pp. e102582, (2014) (PubMed).
抗原

BAK1 (BCL2-Antagonist/killer 1 (BAK1))

别名

BAK (BAK1 产品)

别名

BAK antibody, BAK-LIKE antibody, BCL2L7 antibody, CDN1 antibody, Bak antibody, N-BAK1 antibody, N-Bak antibody, Bak1 antibody, bak antibody, bak1 antibody, BCL2 antagonist/killer 1 antibody, BCL2-antagonist/killer 1 antibody, BCL2 antagonist/killer 1 L homeolog antibody, BAK1 antibody, Bak1 antibody, bak1.L antibody

背景

Synonyms: Apoptosis Regulator Bak, BAK 1, BAK, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK, BAX_MOUSE.
Background: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2.

基因ID

12018

UniProt

O08734

途径

Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response