ERK2 抗体 (Internal Region) (DyLight 680)
Quick Overview for ERK2 抗体 (Internal Region) (DyLight 680) (ABIN6656700)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - Internal Region
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Supplier Product No.
 - 200-344-gq1
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Supplier
 - Rockland
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原理
 - ERK2 Internal Antibody DyLight™680
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交叉反应 (详细)
 - This DyLight™680 conjugated protein A purified mouse monoclonal antibody reacts specifically with human ERK2.
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纯化方法
 - Anti-ERK2 is purified from tissue culture supernatant by protein A purification.
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免疫原
 - Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
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亚型
 - IgG
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Labeling Ratio
 - 3.0
 
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应用备注
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ELISA_Dilution: 1:40,000
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-ERK 2 (MOUSE) DyLight™680 conjugated antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.
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限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 
                                            
储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
 - 12 months
 
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
 - ERK2
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背景
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Synonyms: mouse anti-ERK2 antibody DyLight™680 conjugation, DyLight™680 conjugated mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, DyLight™680, ERK-2 Antibody
Background: ERK2 antibodies detect the ERK2 isoform. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Anti-ERK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
Gene Name: MAPK1
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UniProt
 - P28482
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途径
 - MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
 
抗原
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