p53 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for p53 抗体 (C-Term) (ABIN6294917)
抗原
See all p53 (TP53) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 370-378, C-Term
-
原理
- Mouse anti-Human/Monkey/Dog/Mouse/Rat/Hamster p53 Antibody / TP53 (C-Terminal Region) [Sodium Azide Free]
-
特异性
- Nuclear
-
纯化方法
- SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminal end (aa 370-378) of p53.
-
免疫原
- SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminal end (aa 370-378) of p53.
-
-
anti-Tumor Protein P53 (TP53) antibody
KO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyTP53 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 293-322) antibodyTP53 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7777 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (AA 251-310) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-393) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 378-393), (pSer392) antibodyTP53 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal FP3-2 [FPS392] unconjugated
-
-
应用备注
-
Western blot: 0.5-1 μg/mL
FACS: 0.5-1 μg/million cells
Immunofluorescence: 0.5-1 μg/mL
IHC (Frozen) only: 0.5-1 μg/mL for 30 min at RT -
限制
- 仅限研究用
-
-
-
缓冲液
- In 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
-
-
- p53 (TP53) (Tumor Protein P53 (TP53))
-
别名
- Cellular tumor antigen p53
-
背景
-
Target Description: This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 Molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.
Gene Symbol: TP53///P53
-
基因ID
- 7157
-
UniProt
- P04637
-
途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
-
