AKT1/3 (AA 380-460) 抗体
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- 抗原
- AKT1/3
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抗原表位
- AA 380-460
- 适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit polyclonal to Akt1/3.
- 特异性
- Akt1/3 Polyclonal Antibody detects endogenous levels of Akt1/3 protein.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 免疫原
- Synthesized peptide derived from human Akt1/3 around the non-phosphorylation site of Y437/434.
- 亚型
- IgG
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- 应用备注
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000 - 说明
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Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- 抗原
- AKT1/3
- 分子量
- 50/56 kDa
- 基因ID
- 207
- UniProt
- P31749
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