AKT1/3 (AA 401-479) 抗体
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- 抗原
- AKT1/3
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抗原表位
- AA 401-479
- 适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Dog,Cow,Sheep,Pig,Chicken,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human AKT1
- 亚型
- IgG
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anti-AKT1/3 (pTyr434), (pTyr437) antibody
适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (pTyr437) antibody适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (pTyr437), (Tyr434) antibody适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (pTyr434), (pTyr437) antibody适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (Tyr434), (Tyr437) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (Lys539) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 (AA 406-455), (pTyr437) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1/3 antibody适用: 人 宿主: 小鼠 Monoclonal AT6D5 unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- AKT1/3
- 背景
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Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
- 基因ID
- 207
- UniProt
- P31749
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