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HSP27 抗体 (pSer15)

Name: HSP27 抗体 (pSer15)
SKU: ABIN6267294
Availability: OutOfStock

HSPB1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of HSP27 phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (HSP27 抗体 (pSer15))

ABIN6267294 at 1/200 staining human kidney sections by IHC-P. (HSP27 抗体 (pSer15))

IHC

IF/ICC

3 images

(1 reference)

产品编号 ABIN6267294

发货至: 中国

Datasheet as PDF

抗原 See all HSP27 (HSPB1) 抗体

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
抗原表位
36

36

26

16

16

9

7

7

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer15
适用
285

102

88

24

14

14

13

11

10

5

5

4

3

2

2

1

1
人, 大鼠, 小鼠
宿主
226

63
兔
Compatible Secondaries
克隆类型
202

86
多克隆
标记
127

23

16

13

11

7

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
应用范围
245

103

98

94

66

47

47

41

41

40

15

12

10

7

7

2

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-HSP27 (Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Serine 15.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15.

亚型

IgG

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anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS, pH 7.4,50 % glycerol.

储存条件

-20 °C

有效期

12 months
Xia, Yao, Tang, Xiao, Yang, Zhou: "Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury." in: Frontiers in pharmacology, Vol. 8, pp. 99, (2017) (PubMed).
抗原

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

别名

HSPB1 (HSPB1 产品)

别名

CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

背景

Description: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Gene: HSPB1

分子量

27kDa

基因ID

3315

UniProt

P04792

途径

MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling