ERK2 抗体 (Internal Region)
Quick Overview for ERK2 抗体 (Internal Region) (ABIN6254915)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- ERK2 Antibody detects endogenous levels of total ERK2.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human ERK2, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 200-300) antibody
MAPK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 25-360) antibodyMAPK1 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-360) antibodyMAPK1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1D1 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 9H1 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 6F8 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 9E9 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 200-300) antibodyMAPK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibodyMAPK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 310-360) antibodyMAPK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- MAPK1
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背景
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Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).
Gene: MAPK1
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分子量
- 41kDa
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基因ID
- 5594
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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