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Melanoma gp100 抗体

PMEL 适用: 人, 马 IHC, IF, FACS 宿主: 小鼠 Monoclonal SPM286 unconjugated
产品编号 ABIN6252838
发货至: 中国
  • 抗原 See all Melanoma gp100 (PMEL) 抗体
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    适用
    • 84
    • 12
    • 5
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    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 马
    宿主
    • 46
    • 36
    • 1
    小鼠
    克隆类型
    • 45
    • 38
    • 1
    单克隆
    标记
    • 43
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
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    • 3
    • 2
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    • 1
    • 1
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    This Melanoma gp100 antibody is un-conjugated
    应用范围
    • 38
    • 36
    • 35
    • 34
    • 23
    • 14
    • 6
    • 5
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    特异性
    Does not react with rat
    纯化方法
    Protein G affinity chromatography
    免疫原
    Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.
    克隆位点
    SPM286
    亚型
    IgG2b kappa
    Top Product
    Discover our top product PMEL Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    别名
    gp100 (PMEL 产品)
    别名
    D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
    背景
    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
    基因ID
    6490
    UniProt
    P40967
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