Recombinant Melanoma gp100 抗体
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北京 101111
Quick Overview for Recombinant Melanoma gp100 抗体 (ABIN6940592)
抗原
See all Melanoma gp100 (PMEL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody
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特异性
- The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26kDa and 34 38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
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无交叉反应
- 犬, 大鼠
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human gp100 protein
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亚型
- IgG, kappa
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anti-Premelanosome Protein (PMEL) antibody
PMEL 适用: 人, 大鼠, 猴, 犬 IHC, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 10E5 unconjugated
anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL 适用: 人, 小鼠 WB, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, WB, FACS, IF 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL 适用: 人, 马, 大鼠, 小鼠, 犬, Cow, 山羊, 豚鼠, 兔 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人, 大鼠, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL 适用: 人, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC 宿主: 兔 Monoclonal 617R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (AA 1-661) antibodyPMEL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: SK-MEL-28 cells. Melanoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- SILV
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背景
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95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanosomal matrix protein17, p100, p26, SILV, Silver homolog
COLO-38 or SK-MEL-28 cells. Human melanoma.
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分子量
- 26-100kDa
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基因ID
- 6490, 95972
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UniProt
- P40967
抗原
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