B7-H3 antibody can be used for detection of B7-H3 by Western blot at 0.25 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL and Immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL. Flow cytometry at 1 μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
B7-H3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
B7-H3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 Molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.