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MCAM 抗体

MCAM 适用: 人 FACS, IHC, IF 宿主: 小鼠 Monoclonal C146-634 unconjugated
产品编号 ABIN6151323
发货至: 中国
  • 抗原 See all MCAM 抗体
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    适用
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    宿主
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    小鼠
    克隆类型
    • 87
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    单克隆
    标记
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    This MCAM antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
    原理
    Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), Purified, with BSA
    产品特性
    The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    纯化方法
    Purified
    免疫原
    Recombinant human CD146 protein
    克隆位点
    C146-634
    亚型
    IgG1 kappa
    Top Product
    Discover our top product MCAM Primary Antibody
  • 应用备注
    Immunohistology (formalin)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user
    说明

    A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    200 μg/mL
    缓冲液
    PBS/0.05 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    别名
    CD146 / MCAM / MUC18 / Mucin 18 (MCAM 产品)
    别名
    CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
    分子量
    130 kDa
    基因ID
    4162, 599039
    UniProt
    P43121
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