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NFKB1 抗体 (AA 740-964)

Name: NFKB1 抗体 (AA 740-964)
Brand: antibodies-online
SKU: ABIN6144571
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-NFKB1 antibody is a 兔多克隆 antibody detecting NFKB1 in WB, IHC 和 IF. Suitable for 人. Independently validated for use in Immunofluorescence. This Primary Antibody has been cited in 2+ publications.

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571) (NFKB1 抗体 (AA 740-964))

Western blot analysis of extracts of various cell lines, using NFKB1 antibody. (NFKB1 抗体 (AA 740-964))

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens). (NFKB1 抗体 (AA 740-964))

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody. (NFKB1 抗体 (AA 740-964))

IHC (p)

Immunofluorescence analysis of A549 cells using NFKB1 antibody. (NFKB1 抗体 (AA 740-964))

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens). (NFKB1 抗体 (AA 740-964))

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens). (NFKB1 抗体 (AA 740-964))

IHC

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens). (NFKB1 抗体 (AA 740-964))

8 images

(2 references)

(1 validation)

产品编号 ABIN6144571

发货至: 中国

Quick Overview for NFKB1 抗体 (AA 740-964) (ABIN6144571)

抗原

See all NFKB1 抗体

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This NFKB1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

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AA 740-964

序列

AAGADPLVEN FEPLYDLDDS WENAGEDEGV VPGTTPLDMA TSWQVFDILN GKPYEPEFTS DDLLAQGDMK QLAEDVKLQL YKLLEIPDPD KNWATLAQKL GLGILNNAFR LSPAPSKTLM DNYEVSGGTV RELVEALRQM GYTEAIEVIQ AASSPVKTTS QAHSLPLSPA STRQQIDELR DSDSVCDSGV ETSFRKLSFT ESLTSGASLL TLNKMPHDYG QEGPL

交叉反应

人, 小鼠, 大鼠

产品特性

Polyclonal Antibodies

免疫原

Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).

亚型

IgG
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody
NFKB1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 1-961) antibody
NFKB1 适用: 人 WB, IHC, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody
NFKB1 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 2E6 unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (C-Term) antibody
NFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer927) antibody
NFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibody
NFKB1 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D10D11 unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer893) antibody
NFKB1 适用: 人, 小鼠 ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer337) antibody
NFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibody
NFKB1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D10 unconjugated

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 1-360) antibody
NFKB1 适用: 人 WB, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:100

说明

HIGH QUALITY

限制

仅限研究用
生效 #104072 (Immunofluorescence)

by

Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum

No.

#104072

日期

2019.11.21

抗原

NFKB1

Lot Number

3560449004

Method validated

Immunofluorescence

Positive Control

U-937 human monocytic cell line

Negative Control

No primary antibody control

Notes

Passed. ABIN6144571 specifically stains NF-κB1 in U-937 human monocytic cells.

Validation Images

U937 monocytic cells stained with with anti-NF-κB1/p50 antibody ABIN6144571 and a TRITC-labeled secondary antibody (bottom row, red). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no specific TRITC signal.
Full Methods
Primary Antibody

ABIN6144571

Secondary Antibody

TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.)

Full Protocol

Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO₂ in 300µL in 24-well plates (Sarstedt AG&Co. KG).
Use cytospin to spin cells onto slide.
Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
Wash cells 3x for 5min with PBS.
Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
Incubate cells with primary antibody rabbit anti-human NF-κ1/p50 (antibodies-Online, ABIN6144571, lot 3560449004) diluted 1:100 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
Wash cells 3x for 5min with PBS.
Incubate cells with secondary TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
Wash cells 2x for 5min with PBS.
Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
Image acquisition with an Olympus widefield microscope.

Experimental Notes

Strong red staining dependent on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Du, Qiao, Chen, Chen, Liu, Lin, Wang, Xie: "Toll-Like Receptor 4 Mediates Methamphetamine-Induced Neuroinflammation through Caspase-11 Signaling Pathway in Astrocytes." in: Frontiers in molecular neuroscience, Vol. 10, pp. 409, (2017) (PubMed).

Ye, Chen, Li, Zhao, He, Zohaib, Song, Deng, Zhang, Chen, Cao: "Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).
抗原

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

别名

NFKB1

背景

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.,NFKB1,CVID12,EBP-1,KBF1,NF-kB1,NF-kappa-B,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Signal Transduction,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,NFKB1

分子量

85 kDa/105 kDa

基因ID

4790

UniProt

P19838

途径

p53 Pathway, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins