IL1RN 抗体
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- 抗原 See all IL1RN 抗体
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL1RN antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 产品特性
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Synonyms: IL1RA antibody, IL1RN antibody, IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, rabbit anti-IL-1 Receptor Antagonist Antibody, rabbit anti-1 receptor antagonist protein antibody, rabbit anti-IRAP antibody
Background: Interleukin 1 receptor antagonist is secreted by various types of cells including immune, epithelial and adipocytes. IL 1Ra is used to treat rheumatoid arthritis. Anti-IL-1 RA antibody is ideal for investigators involved in Cancer and Immunology research.
Gene Name: IL1RA
- 纯化方法
- This is an IgG preparation of whole rabbit serum purified by DEAE chromatography. In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes. This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry. When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly.
- 免疫原
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Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL1RN Primary Antibody
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anti-Interleukin 1 Receptor Antagonist (IL1RN) antibody
IL1RN 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 26-177) antibodyIL1RN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 26-177) antibodyIL1RN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 1-159) antibodyIL1RN 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1H5 unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) antibodyIL1RN 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 147-177), (C-Term) antibodyIL1RN 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18902 unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 27-178) antibodyIL1RN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 100-129) antibodyIL1RN 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB18901 unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 24-174) antibodyIL1RN 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor Antagonist (IL1RN) (AA 26-177) antibodyIL1RN 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
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Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown.This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1,000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
- 别名
- IL-1 Receptor Antagonist (IL1RN 产品)
- 别名
- DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody
- 基因ID
- 3557
- UniProt
- P18510
- 途径
- NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
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