ERK2 抗体 (Internal Region)
Quick Overview for ERK2 抗体 (Internal Region) (ABIN5706733)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Internal Region
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Supplier Product No.
- 200-301-gq1
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Supplier
- Rockland
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原理
- ERK2 Internal Antibody
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交叉反应 (详细)
- This protein A purified mouse monoclonal antibody reacts specifically with human ERK2.
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产品特性
- Synonyms: mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 Antibody
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纯化方法
- Anti-ERK2 is purified from tissue culture supernatant by protein A purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: Anti-ERK 2 (MOUSE) antibody has been tested by ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:40,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- MAPK1
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背景
- Background: ERK2 antibodies detect the ERK2 isoform. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Anti-ERK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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