IGFBP5 抗体 (Biotin)
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- 抗原 See all IGFBP5 抗体
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IGFBP5 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Human IGF-BP5 specific antibody was purified by affinity chromatography and then biotinylated.
- Top Product
- Discover our top product IGFBP5 Primary Antibody
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- 应用备注
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ELISA:
Direct:
To detect hIGF-BP5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Sandwich
To detect hIGF-BP5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP5 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Western Blot:
To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存条件
- -20 °C
- 储存方法
- IGF-BP5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
- 别名
- IGF-BP5 (IGFBP5 产品)
- 别名
- IBP5 antibody, AI256729 antibody, AW208790 antibody, IGFBP-5 antibody, IGFBP-5P antibody, IGF-BP5 antibody, igfbp5 antibody, ibp5 antibody, IBP-5 antibody, xIGFBP-5 antibody, IGFBP5 antibody, igfbp5b antibody, zgc:165472 antibody, insulin like growth factor binding protein 5 antibody, insulin-like growth factor binding protein 5 antibody, insulin like growth factor binding protein 5 L homeolog antibody, insulin-like growth factor binding protein 5b antibody, insulin-like growth factor-binding protein 5 antibody, insulin-like growth factor binding protein 5a antibody, IGFBP5 antibody, Igfbp5 antibody, igfbp5.L antibody, igfbp5b antibody, igfbp5 antibody, LOC100194500 antibody, igfbp5a antibody
- 背景
- IGF-BP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFB-P5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGF-BP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.
- 基因ID
- 3488
- UniProt
- P24593
- 途径
- WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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