Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
This antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
免疫原
p56Dok-2 (Phospho-Tyr299) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y (p) -A-V) derived from Human p56Dok-2.
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation