Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
This antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
免疫原
Pyk2 (Phospho-Tyr402) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y (p) -A-E) derived from Human Pyk2.
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.