MAPKAP Kinase 5 抗体 (Internal Region)
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- 抗原 See all MAPKAP Kinase 5 (MAPKAPK5) 抗体
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This MAPKAP Kinase 5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 纯化方法
- MK5 / MAPKAPK5 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 免疫原
- MK5 / MAPKAPK5 Antibody was raised against synthetic peptide PQVLEAQRRHQKEKS found at an internal region of MK5 / MAPKAPK5.
- Top Product
- Discover our top product MAPKAPK5 Primary Antibody
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- 应用备注
- ELISA: antibody detection limit dilution 1:64000. Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8μ,g/mL. Immunoprecipitation:MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11927 or EB11928. Western blots of
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 500 μg/mL
- 缓冲液
- MK5 / MAPKAPK5 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C. Minimize freezing and thawing.
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- 抗原
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- 别名
- MK5 / MAPKAPK5 (MAPKAPK5 产品)
- 别名
- MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody
- 基因ID
- 8550
- NCBI登录号
- NP_003659, NP_620777
- UniProt
- Q8IW41
- 途径
- MAPK Pathway
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