This antibody is expected to recognize all reported isoforms (NP_000556.1, NP_852004.1). The sequence of the immunizing peptide corresponds with a part of the extracellular domain.
纯化方法
Il6ra Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Il6ra Antibody was raised against synthetic peptide YLLQFQLRYRPVWSK found at an internal region of Il6ra.
IL6RA
适用: 人, 小鼠, 大鼠
FACS, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments in Mouse Spleen and Thymus lysates and in lysates of cell lines NIH3T3 and NSO gave no specific signal but low background (at antibody concentration up to 1μ,g/mL).
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Il6ra is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.