Chicken collagen type I 100 % , chicken collagen type II and III <0.1 %, chicken collagen type X <1 %.
交叉反应 (详细)
Chicken
产品特性
Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type II 100 % , no crossreactivity with chicken collagen type I and III, no crossreactivity with human, mouse and rat collagen type II..
纯化方法
Affinity purified
免疫原
Purified collagen type II from fetal chicken cartilage
After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken cartilage,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:200 - 1:600
限制
仅限研究用
状态
Lyophilized
浓度
1 mg/mL
缓冲液
phosphate buffered solution, no BSA and preservative added!