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COL2 抗体

Cited in 7+ publications. The 山羊 多克隆 anti-COL2 antibody (ABIN135048) specifically detects COL2 in WB, ELISA, IP, ICC, IHC (p), IHC (fro) 和 IEM. The antibody is reactive with 人, 小鼠, 大鼠, 兔, 小鸡, Cow, 绵羊 和 Pig samples.
产品编号 ABIN135048
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for COL2 抗体 (ABIN135048)

抗原

See all COL2 抗体
COL2 (Collagen, Type II (COL2))

适用

  • 55
  • 43
  • 38
  • 28
  • 17
  • 12
  • 10
  • 2
  • 2
  • 1
人, 小鼠, 大鼠, 兔, 小鸡, Cow, 绵羊, Pig

宿主

  • 50
  • 16
  • 9
  • 7
山羊

克隆类型

  • 60
  • 22
多克隆

标记

  • 45
  • 11
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This COL2 antibody is un-conjugated

应用范围

  • 54
  • 36
  • 27
  • 21
  • 17
  • 16
  • 15
  • 13
  • 13
  • 13
  • 8
  • 8
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)
  • 原理

    Goat Anti-Type II Collagen-UNLB

    特异性

    Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

    交叉反应 (详细)

    Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

    产品特性

    Purified Anti-Type II Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, and immunoprecipitation assays.

    纯化方法

    Purified by affinity chromatography on type II collagen covalently linked to agarose, cross-adsorbed against Collagen types I, III, IV, V, and VI for minimal reactivity

    免疫原

    Type II collagen

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Pooled antisera from goats hyperimmunized with type II collagen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.4 mg/mL

    缓冲液

    Borate buffered saline, pH 8.2

    储存条件

    4 °C

    储存方法

    2-8°C
  • Tan Timur, Caron, van den Akker, van der Windt, Visser, van Rhijn, Weinans, Welting, Emans, Jahr: "Increased TGF-β and BMP Levels and Improved Chondrocyte-Specific Marker Expression In Vitro under Cartilage-Specific Physiological Osmolarity." in: International journal of molecular sciences, Vol. 20, Issue 4, (2019) (PubMed).

    Kuwahara, Serowoky, Vakhshori, Tripuraneni, Hegde, Lieberman, Crump, Mariani: "Sox9+ messenger cells orchestrate large-scale skeletal regeneration in the mammalian rib." in: eLife, Vol. 8, (2019) (PubMed).

    Fujii, Hirota, Yasoda, Takizawa, Morozumi, Nakamura, Yotsumoto, Kondo, Yamashita, Sakane, Kanai, Ueda, Yamauchi, Yamanaka, Nakao, Kuwahara, Jindo, Furuya, Mashimo, Inagaki, Serikawa, Nakao: "Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death." in: PLoS ONE, Vol. 13, Issue 3, pp. e0194812, (2018) (PubMed).

    Caron, Emans, Sanen, Surtel, Cremers, Ophelders, van Rhijn, Welting: "The Role of Prostaglandins and COX-Enzymes in Chondrogenic Differentiation of ATDC5 Progenitor Cells." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153162, (2016) (PubMed).

    Nakao, Osawa, Yasoda, Yamanaka, Fujii, Kondo, Koyama, Kanamoto, Miura, Kuwahara, Akiyama, Bessho, Nakao: "The Local CNP/GC-B system in growth plate is responsible for physiological endochondral bone growth." in: Scientific reports, Vol. 5, pp. 10554, (2016) (PubMed).

    van den Akker, Surtel, Cremers, Rodrigues-Pinto, Richardson, Hoyland, van Rhijn, Welting, Voncken: "Novel immortal human cell lines reveal subpopulations in the nucleus pulposus." in: Arthritis research & therapy, Vol. 16, Issue 3, pp. R135, (2014) (PubMed).

    Mirmalek-Sani, Orlando, McQuilling, Pareta, Mack, Salvatori, Farney, Stratta, Atala, Opara, Soker: "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering." in: Biomaterials, Vol. 34, Issue 22, pp. 5488-95, (2013) (PubMed).

  • 抗原

    COL2 (Collagen, Type II (COL2))

    别名

    TypeII Collagen

    背景

    Description: Goat Anti-Type II Collagen-UNLB

    NCBI_Official_Symbol: COL2A1

    Synoynms: COL2

    基因ID

    1280

    UniProt

    P02458
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