COL2 抗体
(1 validation)-
- 抗原 See all COL2 抗体
- COL2 (Collagen, Type II (COL2))
-
适用
- 人, 小鼠, 大鼠, Cow, 哺乳动物, 绵羊
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This COL2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
-
Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL2 Primary Antibody
-
anti-Collagen, Type II (COL2) (AA 1201-1300) antibody
COL2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠 WB, ELISA, IHC (p), IHC (fro), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (N-Term) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 1401-1487) antibodyCOL2 适用: 人, 大鼠, 兔 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 101-150) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人, 小鼠, 大鼠 IHC (p) 宿主: 小鼠 Monoclonal 1H1 unconjugated
anti-Collagen, Type II (COL2) antibody (Biotin)COL2 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type II (COL2) antibody (FITC)COL2 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal FITC
anti-Collagen, Type II (COL2) antibody (Biotin)COL2 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
-
- 应用备注
-
Immunohistochemistry Dilution: 1:50 - 1:400
Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:50,000
- 限制
- 仅限研究用
-
- by
- NanoTemper Technologies
- No.
- #104083
- 日期
- 2019.07.23
- 抗原
- COL2
- Lot Number
- 41639
- Method validated
- Unfolding Profile
- Positive Control
- ABIN5596824
- Negative Control
- Notes
Passed. ABIN5596824 showed Ti at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.
- Primary Antibody
- Secondary Antibody
- Full Protocol
- Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #104083 (Unfolding Profile)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.]()
Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.
Full Methods -
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原
- COL2 (Collagen, Type II (COL2))
- 别名
- Collagen Type II (COL2 产品)
- 背景
-
Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody
Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. We extensively purify collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.
Gene Name: COL2A1
- 基因ID
- 1280
- UniProt
- P02458
-
