Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution) (cross-reacting with horse and pig)
交叉反应
马, 猪
交叉反应 (详细)
Cattle, (cross-reacting with horse and pig)
产品特性
Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution).
After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine cartilage,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:100 - 1:500, ELISA ? 1:200 (OD ? 500)
限制
仅限研究用
状态
Lyophilized
浓度
1 mg/mL
缓冲液
phosphate buffered solution, no BSA and preservative added!