COL1A1 抗体 (Biotin)
(4 references)
Quick Overview for COL1A1 抗体 (Biotin) (ABIN5596821)
抗原
See all COL1A1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-103
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Supplier
- Rockland
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原理
- Collagen Type I Antibody Biotin Conjugated
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交叉反应 （详细）
- Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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产品特性
- Synonyms: rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
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纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens.
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免疫原
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Immunogen: Collagen Type I from human and bovine placenta.
Immunogen Type: Native Protein
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亚型
- IgG
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Labeling Ratio
- 10-20
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anti-Collagen, Type I, alpha 1 (COL1A1) (AA 1192-1207), (C-Term) antibody
COL1A1 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 253-471) antibodyCOL1A1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 1225-1453) antibodyCOL1A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 1218-1440) antibodyCOL1A1 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 253-471) antibodyCOL1A1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 100-200) antibodyCOL1A1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) antibody (FITC)COL1A1 适用: 人, 大鼠, 小鼠, Cow WB, IHC, ELISA, IP, FACS 宿主: 兔 Polyclonal FITC
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 1021-1108) antibodyCOL1A1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) (AA 1219-1464) antibodyCOL1A1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7B1G12 unconjugated
anti-Collagen, Type I, alpha 1 (COL1A1) antibody (HRP)COL1A1 适用: 人, 大鼠, 小鼠, Cow WB, IHC, ELISA, IP, FACS 宿主: 兔 Polyclonal HRP
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:3,000 - 1:6,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:3,000 - 1:6,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 90, (2018) (PubMed).
: "Cortactin promotes exosome secretion by controlling branched actin dynamics." in: The Journal of cell biology, Vol. 214, Issue 2, pp. 197-213, (2017) (PubMed).
: "Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis." in: JCI insight, Vol. 1, Issue 4, pp. e83116, (2016) (PubMed).
: "Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." in: Blood, Vol. 122, Issue 7, pp. 1105-13, (2013) (PubMed).
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: "Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 90, (2018) (PubMed).
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- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
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别名
- COL1A1
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背景
- Background: COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
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基因ID
- 1277
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NCBI登录号
- NP_000079
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UniProt
- P02452
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途径
- Sensory Perception of Sound, Autophagy, Growth Factor Binding
抗原
-
