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His Tag 抗体 (N-Term) (Biotin)

Name: His Tag 抗体 (N-Term) (Biotin)
SKU: ABIN5579852
Availability: OutOfStock

WB, ELISA, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 Biotin

Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) at 0. (His Tag 抗体 (N-Term) (Biotin))

ELISA

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) . (His Tag 抗体 (N-Term) (Biotin))

IHC

3 images

产品编号 ABIN5579852

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag
抗原表位
16

2

1
N-Term

适用

请咨询
宿主
93

55

12

9

8

7

2

1
兔
Compatible Secondaries
克隆类型
105

69

10
单克隆
标记
91

22

14

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to Biotin
应用范围
152

102

37

27

25

16

14

14

13

13

13

7

7

6

5

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Recombinant rabbit monoclonal antibody raised against His-Tag.

免疫原

Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.

克隆位点

RM146

亚型

IgG

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anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

应用备注

ELISA (0.01-0.5 μg/mL)
Flow Cytometry (0.5-2 μg/mL)
Immunocytochemistry (0.5-2 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 μg/mL)
Immunoprecipitation
Western Blotting (0.1-0.5 μg/mL)
The optimal wor

限制

仅限研究用
状态

Liquid

缓冲液

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
抗原

His Tag

别名

His-Tag (His Tag 产品)

物质类

Tag