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His Tag 抗体 (N-Term)

Name: His Tag 抗体 (N-Term)
SKU: ABIN5579851
Availability: OutOfStock

WB, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 unconjugated

Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E. (His Tag 抗体 (N-Term))

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 . (His Tag 抗体 (N-Term))

IHC

Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 . (His Tag 抗体 (N-Term))

3 images

产品编号 ABIN5579851

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag
抗原表位
16

2

1
N-Term

适用

请咨询
宿主
93

55

12

9

8

7

2

1
兔
Compatible Secondaries
克隆类型
105

69

10
单克隆
标记
90

22

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
152

103

37

27

25

16

14

14

13

13

13

7

7

6

5

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Recombinant rabbit monoclonal antibody raised against of His-Tag.

特异性

This antibody reacts to recombinant proteins containing the 6X His-Tag or 10X His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

免疫原

Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.

克隆位点

RM146

亚型

IgG

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应用备注

Flow Cytometry (0.5 μg/mL-2 μg/mL)
Immunocytochemistry (0.5 μg/mL-2 μg/mL)
Immunohistochemistry (0.1 μg/mL-1 μg/mL)
Immunoprecipitation (0.5 μg/mL-2 μg/mL)
Western Blot (0.1 μg/mL-1 μg/mL)
The optimal working dilution should be determined b

限制

仅限研究用
状态

Liquid

缓冲液

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
抗原

His Tag

别名

His-Tag (His Tag 产品)

物质类

Tag