His Tag 抗体
(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays.
Here are some common applications of antibodies that detect His tags:
- Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
- Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.
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His Tag 抗体 by Grade
Find His Tag 抗体 with a specific Grade. The Grade listed below are among those available. Click on a link to go to the corresponding products.
His Tag 抗体 by 适用
Find His Tag 抗体 for a variety of species such as anti-Tag His Tag, anti-Human His Tag, anti-Various Species His Tag. The species listed below are among those available. Click on a link to go to the corresponding products.
His Tag 抗体 by 抗体来源
Find His Tag 抗体 with a specific 抗体来源. The 抗体来源 listed below are among those available. Click on a link to go to the corresponding products.
His Tag 抗体 by 抗原表位
Find His Tag 抗体 with a specific epitope. The epitopes listed below are among those available. Click on a link to go to the corresponding products.
His Tag 抗体 by 克隆形成能力
Find available monoclonal or polyclonal His Tag 抗体. Click on a link to go to the corresponding products.
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Latest Publications for our His Tag 抗体
: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).
: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).
: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).
: "Intellectual disability-associated UNC80 mutations reveal inter-subunit interaction and dendritic function of the NALCN channel complex." in: Nature communications, Vol. 11, Issue 1, pp. 3351, (2020) (PubMed).
: "Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain." in: Acta neuropathologica communications, Vol. 6, Issue 1, pp. 25, (2019) (PubMed).
: "Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist." in: The Journal of clinical investigation, Vol. 129, Issue 4, pp. 1713-1726, (2019) (PubMed).
: "B-cell depletion induces a shift in self antigen specific B-cell repertoire and cytokine pattern in patients with bullous pemphigoid." in: Scientific reports, Vol. 9, Issue 1, pp. 3525, (2019) (PubMed).
: "Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia." in: Infection and immunity, Vol. 87, Issue 6, (2019) (PubMed).
: "HIV Infection Induces Extracellular Cathepsin B Uptake and Damage to Neurons." in: Scientific reports, Vol. 9, Issue 1, pp. 8006, (2019) (PubMed).
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