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His Tag 抗体 (DyLight 649)

WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649
产品编号 ABIN6720637
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
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    小鼠
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    单克隆
    标记
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    This His Tag antibody is conjugated to DyLight 649
    应用范围
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    Western Blotting (WB), Fluorescence Microscopy (FM), FLISA
    纯化方法
    This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
    免疫原

    Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

    Immunogen Type: Peptide

    克隆位点
    33D10-D2
    亚型
    IgG1
    Labeling Ratio
    2.5
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注

    Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000-1:25,000

    IF Microscopy Dilution: >1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
  • Kariolis, Miao, Diep, Nash, Olcina, Jiang, Jones, Kapur, Mathews, Koong, Rankin, Cochran, Giaccia: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." in: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).

    Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).

  • 抗原
    His Tag
    别名
    6X HIS tagged (His Tag 产品)
    物质类
    Tag
    背景

    Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

    Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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