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His Tag 抗体

WB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
产品编号 ABIN7131650
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
    • 85
    • 53
    • 11
    • 6
    • 5
    • 4
    • 1
    • 1
    小鼠
    克隆类型
    • 86
    • 67
    • 10
    单克隆
    标记
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    This His Tag antibody is un-conjugated
    应用范围
    • 131
    • 84
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    Western Blotting (WB), ELISA
    纯化方法
    Protein A purified
    免疫原
    HHHHHH(6x His) synthetic peptide conjugate to KLH
    克隆位点
    3G5H8
    亚型
    IgG2a
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    Recommended dilution: WB:1:500-1:2000,
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Preservative: 0.03 % Proclin 300
    Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C,-80 °C
    储存方法
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 抗原
    His Tag
    别名
    6*His (His Tag 产品)
    物质类
    Tag
    背景

    Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

    Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

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